Coding

Part:BBa_K5213002:Design

Designed by: Patrik Hains   Group: iGEM24_Lund   (2024-08-17)


VDBP1 [Corallina pilulifera]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1808
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 133
    Illegal SapI.rc site found at 1330
    Illegal SapI.rc site found at 1738


Design Notes

Codon harmonized for expression in C.necator; 6xHis Tag added to 5' end (after start codon); stop codon changed to TAATAA


Source

Corallina pilulifera, D87657 [GenBank nucletide ID], BAA31261 [GenBank protein ID];

Frank et al. (2016) - Characterization of a Cyanobacterial Haloperoxidase and Evaluation of its Biocatalytic Halogenation Potential

References